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superscript ii rnase h2 reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher superscript ii rnase h2 reverse transcriptase
    Superscript Ii Rnase H2 Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+rnase+h2+reverse+transcriptase/Ribonuclease+A/pm41316283-49-17-23
    Average 99 stars, based on 1 article reviews
    superscript ii rnase h2 reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: The effect of ABRE BINDING FACTOR 4-mediated FYVE1 on salt stress tolerance in Arabidopsis.
    Article Snippet: FYVE1 encodes a protein that is localized to the peripheral membrane of late endosomal compartments, and is involved in the regulation of mulitivesicular/prevacuolar compartment protein sorting.. It was found that FYVE1 attenuates ABA signaling through degrading ABA receptors PYR1 and PYL4 by ESCRT pathway, and also interacts with transcription factors ABF4 and ABI5 to transcriptionally inhibit ABA signaling pathway by reducing their binding to the cis-regulatory sequences of their downstream genes.. However, the mechanisms underlying the transcriptional regulation of FYVE1 and its biological function in salt stress are largely unknown.

    Article Title: CDR1, a DUF946 domain containing protein, positively regulates cadmium tolerance in Arabidopsis thaliana by maintaining the stability of OPT3 protein.
    Article Snippet: • A novel positive regulatory gene, CDR1, involved in Cd stress response was identified in Arabidopsis.. • CDR1 was confirmed to positively regulate Cd stress tolerance and affect the distribution of Cd in Arabidopsis.. • CDR1 interacts with OPT3 and maintains its protein stability.

    Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage.
    Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [19]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of SuperScript II RNase H2 reverse transcriptase (Invitrogen). cDNA synthesis was primed with primers 7500 A and Q8 [20], and conducted at 42 °C for 2 h, followed by a 15 min incubation at 60 °C to inactivate the enzyme. .. Finally, the RNA in the RNA/DNA hybrid was specifically degraded with 1 μL of RNase H (Promega) at 37 °C for 30 min.

    Article Title: A MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis
    Article Snippet: .. Plant total RNA was extracted from 2-week-old seedlings using Trizol Reagent (Invitrogen, CA, USA), and the first-stand cDNA was synthesized by total RNA with SuperScript II RNase H2 reverse transcriptase (Invitrogen) using random hexamer primer (Promega). ..

    Reverse Transcription:

    Article Title: The effect of ABRE BINDING FACTOR 4-mediated FYVE1 on salt stress tolerance in Arabidopsis.
    Article Snippet: FYVE1 encodes a protein that is localized to the peripheral membrane of late endosomal compartments, and is involved in the regulation of mulitivesicular/prevacuolar compartment protein sorting.. It was found that FYVE1 attenuates ABA signaling through degrading ABA receptors PYR1 and PYL4 by ESCRT pathway, and also interacts with transcription factors ABF4 and ABI5 to transcriptionally inhibit ABA signaling pathway by reducing their binding to the cis-regulatory sequences of their downstream genes.. However, the mechanisms underlying the transcriptional regulation of FYVE1 and its biological function in salt stress are largely unknown.

    Article Title: CDR1, a DUF946 domain containing protein, positively regulates cadmium tolerance in Arabidopsis thaliana by maintaining the stability of OPT3 protein.
    Article Snippet: • A novel positive regulatory gene, CDR1, involved in Cd stress response was identified in Arabidopsis.. • CDR1 was confirmed to positively regulate Cd stress tolerance and affect the distribution of Cd in Arabidopsis.. • CDR1 interacts with OPT3 and maintains its protein stability.

    Article Title: Thioredoxin h2 contributes to the redox regulation of mitochondrial photorespiratory metabolism.
    Article Snippet: Max-Planck Partner Group at the Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900, Viçosa, Minas Gerais, Brazil Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, 60451970, Fortaleza, Ceará, Brazil Ludwig-Maximilians-Universität München, Department Biology I, 82152 PlaneggMartinsried, Germany University of Rostock, Plant Physiology Department, AlbertEinstein-Straße 3, D-18051 Rostock, Germany Max-Planck-Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany

    Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage.
    Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [19]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of SuperScript II RNase H2 reverse transcriptase (Invitrogen). cDNA synthesis was primed with primers 7500 A and Q8 [20], and conducted at 42 °C for 2 h, followed by a 15 min incubation at 60 °C to inactivate the enzyme. .. Finally, the RNA in the RNA/DNA hybrid was specifically degraded with 1 μL of RNase H (Promega) at 37 °C for 30 min.

    Article Title: A MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis
    Article Snippet: .. Plant total RNA was extracted from 2-week-old seedlings using Trizol Reagent (Invitrogen, CA, USA), and the first-stand cDNA was synthesized by total RNA with SuperScript II RNase H2 reverse transcriptase (Invitrogen) using random hexamer primer (Promega). ..

    Article Title: The interplay between autophagy and chloroplast vesiculation pathways under dark-induced senescence
    Article Snippet: The integrity of the RNA was checked on 1% (w/v) agarose gels, and the concentration was measured using a Nanodrop spectrophotometer. .. Finally, 2 μg of total RNA were reverse transcribed with Superscript II Rnase H2 reverse transcriptase (Invitrogen) and oligo (dT) primer according to the manufacturer’s recommendations. .. Real-time PCR reactions were performed in a 96-well microplate (Applied Biosystems Applera, Darmstadt, Germany), using Power SYBR Green PCR Master Mix.

    Article Title: Coordination between GROWTH-REGULATING FACTOR1 and GRF-INTERACTING FACTOR1 plays a key role in regulating leaf growth in rice
    Article Snippet: .. For quantitative RT-PCR analysis of OsGRF1 (Gene ID: Os02g0776900), OsGIF1 (Gene ID: Os03g52320), cycOs1 (Gene ID: Os04g0563700) and cycOs2 (Gene ID: Os06g0726800), 2 μg of total RNA was reversely transcribed in a total volume of 20 μL with 0.5 mg oligo (dT)15, 0.75 mM dNTPs, 10 mM DTT, and 100 U SuperScript II RNase H2 reverse transcriptase (Invitrogen). ..

    Article Title: Molecular manipulations of miR398 increase rice grain yield under different conditions
    Article Snippet: .. For quantitative RT-PCR analysis of OsCSD1 ( Os03g22810 ), OsCSD2 ( Os07g46990 ), and a Copper Chaperone for Cu/Zn Superoxide Dismutase ( CCS1 , Os04g48410 ), 2 μg of total RNA was reversely transcribed in a total volume of 20 μL with 0.5 mg oligo (dT)15, 0.75 mM dNTPs, 10 mM DTT, and 100 U SuperScript II RNase H2 reverse transcriptase (Invitrogen). ..

    Random Hexamer:

    Article Title: The effect of ABRE BINDING FACTOR 4-mediated FYVE1 on salt stress tolerance in Arabidopsis.
    Article Snippet: FYVE1 encodes a protein that is localized to the peripheral membrane of late endosomal compartments, and is involved in the regulation of mulitivesicular/prevacuolar compartment protein sorting.. It was found that FYVE1 attenuates ABA signaling through degrading ABA receptors PYR1 and PYL4 by ESCRT pathway, and also interacts with transcription factors ABF4 and ABI5 to transcriptionally inhibit ABA signaling pathway by reducing their binding to the cis-regulatory sequences of their downstream genes.. However, the mechanisms underlying the transcriptional regulation of FYVE1 and its biological function in salt stress are largely unknown.

    Article Title: CDR1, a DUF946 domain containing protein, positively regulates cadmium tolerance in Arabidopsis thaliana by maintaining the stability of OPT3 protein.
    Article Snippet: • A novel positive regulatory gene, CDR1, involved in Cd stress response was identified in Arabidopsis.. • CDR1 was confirmed to positively regulate Cd stress tolerance and affect the distribution of Cd in Arabidopsis.. • CDR1 interacts with OPT3 and maintains its protein stability.

    Article Title: A MYB4-MAN3-Mannose-MNB1 signaling cascade regulates cadmium tolerance in Arabidopsis
    Article Snippet: .. Plant total RNA was extracted from 2-week-old seedlings using Trizol Reagent (Invitrogen, CA, USA), and the first-stand cDNA was synthesized by total RNA with SuperScript II RNase H2 reverse transcriptase (Invitrogen) using random hexamer primer (Promega). ..

    Purification:

    Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage.
    Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [19]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of SuperScript II RNase H2 reverse transcriptase (Invitrogen). cDNA synthesis was primed with primers 7500 A and Q8 [20], and conducted at 42 °C for 2 h, followed by a 15 min incubation at 60 °C to inactivate the enzyme. .. Finally, the RNA in the RNA/DNA hybrid was specifically degraded with 1 μL of RNase H (Promega) at 37 °C for 30 min.

    cDNA Synthesis:

    Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage.
    Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [19]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of SuperScript II RNase H2 reverse transcriptase (Invitrogen). cDNA synthesis was primed with primers 7500 A and Q8 [20], and conducted at 42 °C for 2 h, followed by a 15 min incubation at 60 °C to inactivate the enzyme. .. Finally, the RNA in the RNA/DNA hybrid was specifically degraded with 1 μL of RNase H (Promega) at 37 °C for 30 min.

    Incubation:

    Article Title: Characterization of a Sabin-like type 2/3 recombinant poliovirus with a triple nucleotide deletion in the VP1 region isolated from sewage.
    Article Snippet: Viral RNA was extracted from poliovirus isolates using the QIAamp Mini Viral RNA Extraction Kit (Qiagen) and subsequently used for RT-PCR amplification, following standard protocols [19]. .. Single-stranded cDNA was synthesized from 5 μL of purified viral RNA using 1 μL (200 U) of SuperScript II RNase H2 reverse transcriptase (Invitrogen). cDNA synthesis was primed with primers 7500 A and Q8 [20], and conducted at 42 °C for 2 h, followed by a 15 min incubation at 60 °C to inactivate the enzyme. .. Finally, the RNA in the RNA/DNA hybrid was specifically degraded with 1 μL of RNase H (Promega) at 37 °C for 30 min.

    Quantitative RT-PCR:

    Article Title: Coordination between GROWTH-REGULATING FACTOR1 and GRF-INTERACTING FACTOR1 plays a key role in regulating leaf growth in rice
    Article Snippet: .. For quantitative RT-PCR analysis of OsGRF1 (Gene ID: Os02g0776900), OsGIF1 (Gene ID: Os03g52320), cycOs1 (Gene ID: Os04g0563700) and cycOs2 (Gene ID: Os06g0726800), 2 μg of total RNA was reversely transcribed in a total volume of 20 μL with 0.5 mg oligo (dT)15, 0.75 mM dNTPs, 10 mM DTT, and 100 U SuperScript II RNase H2 reverse transcriptase (Invitrogen). ..

    Article Title: Molecular manipulations of miR398 increase rice grain yield under different conditions
    Article Snippet: .. For quantitative RT-PCR analysis of OsCSD1 ( Os03g22810 ), OsCSD2 ( Os07g46990 ), and a Copper Chaperone for Cu/Zn Superoxide Dismutase ( CCS1 , Os04g48410 ), 2 μg of total RNA was reversely transcribed in a total volume of 20 μL with 0.5 mg oligo (dT)15, 0.75 mM dNTPs, 10 mM DTT, and 100 U SuperScript II RNase H2 reverse transcriptase (Invitrogen). ..



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